Description and Validation of a Novel Real-Time RT-PCR Enterovirus Assay
نویسندگان
چکیده
منابع مشابه
Description and validation of a novel real-time RT-PCR enterovirus assay.
BACKGROUND Enteroviruses are a leading cause of aseptic meningitis in adult and pediatric populations. We describe the development of a real-time RT-PCR assay that amplifies a small target in the 5' nontranslated region upstream of the classical Rotbart enterovirus amplicon. The assay includes an RNA internal control and incorporates modified nucleotide chemistry. METHODS We evaluated the per...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2008
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2007.095414